Accession Number : ADA265460


Title :   Antigenicity of Campylobacter Jejuni Flagella


Corporate Author : VETERANS ADMINISTRATION MEDICAL CENTER DENVER CO


Personal Author(s) : Blaser, Martin J ; Hopkins, Janet A ; Perez-Perez, Guillermo I ; Cody, Henry J ; Newell, Diane G


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a265460.pdf


Report Date : 28 Mar 1986


Pagination or Media Count : 7


Abstract : We studied the antigenicity of a wild-type flagellate and motile F(+) M(+) Campylobacter jejuni strain (81116) and two daughter mutants, one flagellate and immotile F (+)M(-) and one aflagellate and immotile F(-)M(-). By solium dodecyl sulfate-polyacrylamide gel electrophoresis of acid-extracted surface proteins, a 63-kilodalton (kDa) band identified from sheared flagella as the flagellar protein was present in the F(+)M(+) and F(+)M(-) strains but not in the F(-)M(-) strain. No other differences in protein profile among the three strains were noted. By Western blotting, serum from rabbits immunized with either the F(+)M(+) or F(-)M(-) strain detected a 63-kDa protein in the F(+)M(+) and F(+)M(-) strains but not in the F(-)M(-) strain. That the F(-)M(-) antiserum recognized the 63-kDa band suggests that small amounts of this protein or a cross-reacting antigen is present on the F(-)M(-) strain. By counterimmunoelectrophoresis of the acid-extracted preparations with immune sera, all three strains were found to share three major antigens, but a fourth antigen with a net positive charge was present only in the F(+)M(+) and F(+)M(-) strains. Antisera to five C. jejuni and two Campylobacter fetus strains recognized the 63-kDa protein oif purified F(+)M(+) flagella in Western blots, demonstrating that a common antigen is present, but enzyme-linked immunosorbent assay results suggest that the sharing of this antigen among Campylobacter strains is variable


Descriptors :   *ANTIGENS , MEMBRANES(BIOLOGY) , HUMANS , BACTERIA , DIARRHEA , STRAINS(BIOLOGY)


Subject Categories : Medicine and Medical Research
      Microbiology


Distribution Statement : APPROVED FOR PUBLIC RELEASE