Accession Number : ADA264438


Title :   Molecular Approach to Hypothalamic Rhythms: Isolation of Novel Indoleamine Receptor Genes


Descriptive Note : Annual rept. 15 Mar 1992-14 Mar 1993


Corporate Author : SCRIPPS RESEARCH INST LA JOLLA CA


Personal Author(s) : Sutcliffe, J G ; Erlander, Mark G ; Lovenberg, Timothy W ; Baron, Bruce M ; de Lecea, Luid


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a264438.pdf


Report Date : 14 Mar 1993


Pagination or Media Count : 25


Abstract : We have utilized polymerase chain reaction with primers corresponding to conserved amino acid sequences within membrane-spanning regions of known serotonin receptors to identify clones of 4 putative new indoleamine receptors. We have determined complete amino acid sequences of these 4 receptors which fall into 3 subfamilies; two of these subfamilies are novel. The sites of expression within the brain have been determined for each of the genes. Expression in mammalian cells demonstrates that each new protein is a receptor for serotonin and each has a distinct pharmacology when compared to known receptors. Two of the new receptors are coupled to CAMP, one negatively (G) and one positively (Gs). The latter is a candidate for the serotonin receptor that mediates phase advances in circadian rhythms of the SCN.


Descriptors :   *SEROTONIN , *CHAIN REACTIONS , BRAIN , CIRCADIAN RHYTHMS , PROTEINS , SEQUENCES , AMINO ACIDS , PHARMACOLOGY , CELLS(BIOLOGY) , PRIMERS , MEMBRANES , GENES , CLONES


Subject Categories : Biochemistry
      Pharmacology


Distribution Statement : APPROVED FOR PUBLIC RELEASE