Accession Number : ADA264059


Title :   Polymerase Chain Reaction Assays For Diagnosis of Pneumocystis Carinii Infections


Descriptive Note : Final rept. 1 Feb 91-31 Jan 93,


Corporate Author : ARMED FORCES INST OF PATHOLOGY WASHINGTON DC


Personal Author(s) : O'Leary, Timothy J


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a264059.pdf


Report Date : 25 Mar 1993


Pagination or Media Count : 32


Abstract : The use of quantitative polymerase chain reaction (QPC ) for assessing PCP infection and its response to treatment was studied in rats. Groups of 8 rats were immunosuppressed with steroids for 3-12 weeks. Controls were maintained untreated for the same periods. Three groups of rats were treated with pentamadine, and three additional groups with trimethoprim and sulfamethoxazole while immunosuppressed. Three groups of rats were tapered from steroids. At various times during suppression the rats were sacrificed. At necropsy the lungs were lavaged and the BAL fluid assayed by cyst counting and QPCR using ribosomal DNA to quantitate PCP load in using a rat globin internal standard. Lung homogenates were similarly assayed. An increase in the QPCR signal was seen throughout immunosuppression, followed by a slow decrease upon withdrawal of steroids or a faster decrease with drug treatment. Results from QPCR of lung and BAL fluid were strongly correlated with each other and with cyst counts. 'Results warrant investigation of QPCR for assessing treatment results in human Pcp infection.... Pneumocystis carinii, Polymerase chain reaction diagnosis, HIV, Diagnostic assay.


Descriptors :   *HUMAN IMMUNODEFICIENCY VIRUSES , *ASSAYING , *PNEUMOCYSTIS CARINII , CONTROL , RATS , DIAGNOSIS(MEDICINE) , CHAIN REACTIONS , IMMUNOSUPPRESSION , PROTEINS(SIMPLE) , CYSTS , STEROIDS , BLOOD , LUNG , FLUIDS , RESPONSE(BIOLOGY) , DRUGS


Subject Categories : Anatomy and Physiology
      Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE