Accession Number : ADA263043


Title :   The Kappa Opioid Agonist U-50, 488H Antagonizes Respiratory Effects of Mu Opioid Receptor Agonists in Conscious Rats


Corporate Author : WALTER REED ARMY INST OF RESEARCH WASHINGTON DC


Personal Author(s) : Dosaka-Akita, Kumi ; Tortella, Frank C ; Holaday, John W ; Long, Joseph B


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a263043.pdf


Report Date : Jan 1993


Pagination or Media Count : 8


Abstract : The interactive effects of mu and kappa opioid receptor agonists on respiratory function were investigated following their i.c.v. injection into conscious rats. The highly selective mu receptor agonist (D-Ala(2), N-Methyl- Phe(4), Gly-ol) enkephalin (DAMGO; 1.2-10 nmol) and the relatively selective mu agonist morphine (20 and 30 nmol) significantly decreased arterial pH and PO2, and increased arterial PCO2 and blood pressure. Morphine and a low dose of DAMGO (1.2 nmol) also significantly elevated respiratory rate. Heart rate was decreased by DAMGO and, depending upon dose, was either decreased (20 nmol) or increased (30 nmol) by morphine. The selective kappa opioid agonist U-50, 488H (200 nmol i.c.v.), which by itself had no significant effect on either respiration or cardiovascular function, dose-dependently antagonized the acidotic, hypoxemic and hypercapnic effects of both DAMGO (2.5 nmol) and morphine (30 nmol). Furthermore, these mu antagonistic properties of U-50, 488H were blocked completely after pretreatment with 25 nmol of the highly selective kappa opioid antagonist nor-binaltorphimine. These results indicate that the antagonism of mu opioid respiratory depressant effects by U-50, 488H is kappa opioid receptor mediated.


Descriptors :   *RESPIRATION , *RECEPTOR SITES(PHYSIOLOGY) , *MORPHINE , *PHARMACOLOGICAL ANTAGONISTS , REPRINTS , INJECTION , IN VIVO ANALYSIS , BLOOD PRESSURE , PHARMACOLOGY , HEART RATE , DOSE RATE , RATS , AVAILABILITY


Subject Categories : Biochemistry
      Pharmacology


Distribution Statement : APPROVED FOR PUBLIC RELEASE