Accession Number : ADA262447


Title :   Hepatic Metabolism of Perfluorinated Carboxylic Acids and Polycholorotrifluoroethylene: A Nuclear Magnetic Resonance Investigation in Vivo


Descriptive Note : Annual technical rept. 15 Dec 91-14 Dec 92


Corporate Author : WRIGHT STATE UNIV DAYTON OH SCHOOL OF MEDICINE


Personal Author(s) : Reo, Nicholas V


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a262447.pdf


Report Date : 14 Jan 1993


Pagination or Media Count : 24


Abstract : This report describes our studies of the effects of perfluorooctanoic acid (PFOA) and perfluorodecanoic acid (PFDA) on hepatic carbohydrate and phospholipid metabolism. Previously we have shown that PFDA inhibits hepatic glycogenesis from glucose in rats. In recent studies using carbon-13 nuclear magnetic resonance (NMR) spectroscopy, PFDA-treated rats show active gluconeogenesis from (3-13) Calanine and the incorporation of the 13C label into hepatic glycogen. The rate of alanine utilization is 40% greater in controls than PFDA-treated rats, and liver glucose-6-phosphate levels are about 40% lower in PFDA rats as compared to controls (p or = 0.02). These results suggest that the PFDA-induced inhibition in glycogenesis from glucose is due to a dysfunction in the glucose transporter and/or glucoinase activity. In separate studies involving liver phosphorus metabolism, 31 P NMR was used to examine the effects of PFDA, PFOA, and clofibrate (CLOF) in both rats and guinea pigs. A unique effect is revealed in PFDA-treated rats in which a significant increase is observed in liver phosphocholine from 2.31 +/- 0.23 umol/g tissue on day 1 post dose to 4.56 +/- 0.21 umol/g on day 5. These level are 2 to 4-fold greater than those measured in controls. The results indicate an enhanced turnover of liver phosphatidylcholine. Ongoing research efforts focus on the effects of PFDA on diacylglycerol levels, phospholipase C activity, and will examine the physical interaction of PFDA with phospholipid membranes.


Descriptors :   *NUCLEAR MAGNETIC RESONANCE , *CARBOXYLIC ACIDS , RATS , SPECTROSCOPY , INTERACTIONS , RATES , CARBON , METABOLISM , SPECTRA , SAMPLING , MEMBRANES , LIVER , INHIBITION , IN VIVO ANALYSIS , CARBOHYDRATES , UTILIZATION , BIOSYNTHESIS , METABOLITES , GLUCOSE , DYSFUNCTION , LABELS , BLOOD PLASMA , PHOSPHATES , LABORATORY ANIMALS , GUINEA PIGS , ISOTOPE EFFECT , PHOSPHOLIPIDS , BILE , ALANINES , GLYCOGEN


Subject Categories : Biochemistry


Distribution Statement : APPROVED FOR PUBLIC RELEASE