Accession Number : ADA262274


Title :   The Molecular Anatomy of PFDA Hepatotoxicity as Studied by Two-Dimensional Electrophoresis


Descriptive Note : Final rept. 15 Dec 1989-14 Dec 1992


Corporate Author : INDIANA UNIV-PURDUE UNIV AT COLUMBUS DEPT OF BIOLOGY


Personal Author(s) : Witzmann, Frank A


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a262274.pdf


Report Date : 26 Feb 1993


Pagination or Media Count : 24


Abstract : Perfluoro-n-decanoic acid (PFDA) effects on protein expression in the rat liver were studied in rodents following In vivo exposure to PFDA levels above, below and at the LD-50. Two-dimensional whole-liver homogenate protein patterns were generated and compared to previous results. As before, numerous proteins were altered; some suppressed, some induced, but most were unaffected. In an effort to identify the altered proteins, further analysis of basic proteins by first-dimension NEPHGE revealed the induction of cytochrome P452 (lauric acid omega-oxidase) and enoyl-CoA hydratase. Induction of these and related enzymes confirms previously observed PFDA-induced peroxisome proliferation and lends strong support to the notion that PFDA blocks normal Beta-oxidation, causes fatty acid accumulation, and results in compensatory peroxisomal and mitochondrial omega- and Beta-oxidation Continued identification of other altered proteins will be undertaken to add to the metabolic paths affected by PFDA to further delineate its toxic mechanism.... Rat liver, Perfluoro-n-decanoic acid, Hepatotoxicity, Two-dimensional electrophoresis, Peroxisome proliferation, Image analysis.


Descriptors :   *LIVER , *ELECTROPHORESIS , RATS , MOLECULES , TWO DIMENSIONAL , TOXICITY , ENZYMES , PROTEINS , METABOLISM , OXIDATION , IMAGES , TOXIC AGENTS , PATTERNS , ANATOMY , ACCUMULATION , FATTY ACIDS , OXIDOREDUCTASES


Subject Categories : Toxicology


Distribution Statement : APPROVED FOR PUBLIC RELEASE