Accession Number : ADA261008


Title :   Verification, Dosimetry and Biomonitoring of Mustard Gas Exposure via Immunochemical Detection of Mustard Gas Adducts to DNA and Proteins


Descriptive Note : Final rept. 15 May 1988-14 May 1991


Corporate Author : PRINS MAURITS LABORATORIUM TNO RIJSWIJK(NETHERLANDS)


Personal Author(s) : Benschop, Hendrik P


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a261008.pdf


Report Date : Dec 1991


Pagination or Media Count : 298


Abstract : The use of mustard gas in the Iran-Iraq War has stressed the need of reliable methods for retrospective detection of poisoning with chemical warfare agents. We are developing methods to detect adducts of mustard gas to DNA and proteins, which may have a life span of many days after exposure. In order to achieve high sensitivity and specificity, immunochemical detection techniques are developed. As markers to detect adducts, we synthesized N7-(2'- hydroxyethylthioethyl)-guanine, the di-adduct di-(2-guanin-7'-yl-ethyl) sulfide, and N3-(2'-hydroxyethylthioethyl-adenine, 06-(2'-hydroxyethylthioethyl)-guanine and the corresponding 2'deoxyguanosine derivative. N7-(2'-hydroxyethylthioethyl) -guanine was shown to be the major adduct in experiments with double-stranded calf-thymus DNA and human white blood cells exposed to (355)-mustard gas. The N7-guanine di-adduct and the N3-adenine adduct were formed to a lesser extent, whereas the 06-guanine adduct was not detected.... Mustard Gas, Mustard Gas (HD) adducts, Mustard Gas exposure, Mustard Gas DNA-Adducts, Mustard Gas DNA-adducts in human blood, Mustard Gas protein-adducts.


Descriptors :   *SENSITIVITY , *POISONING , *MARKERS , *MUSTARD AGENTS , *CHEMICAL WARFARE AGENTS , METHODOLOGY , DETECTION , BIOLOGY , HUMANS , PROTEINS , DEOXYRIBONUCLEIC ACIDS , RELIABILITY , EXPOSURE(PHYSIOLOGY) , LIFE SPAN(BIOLOGY) , SULFIDES , LEUKOCYTES , IMMUNOCHEMISTRY , BLOOD CELLS , HIGH SENSITIVITY , ADENINE , GUANINE , THYMUS


Subject Categories : Biochemistry
      Chemical, Biological and Radiological Warfare


Distribution Statement : APPROVED FOR PUBLIC RELEASE