Accession Number : ADA260826


Title :   0.3 MM Diameter Flexible Amperometric Lactate Probe.


Descriptive Note : Technical rept. Oct 92-Nov 93,


Corporate Author : TEXAS UNIV AT AUSTIN DEPT OF CHEMICAL ENGINEERING


Personal Author(s) : Wang, Dan L ; Heller, Adam


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a260826.pdf


Report Date : 26 Jan 1993


Pagination or Media Count : 32


Abstract : A flexible lactate electrode was made of 400 plus or minus 100 7 micrometers diameter carbon fibers, epoxy embedded in a 0.3 mm diameter polyimide tubing. The electrode was modified by precipitating on it the relatively insoluble complex formed between 1100 kd partially N-ethylamine quaternized poly((vinylpyridine)Os(bipyridine)2Cl)Cl (POs-EA) and lactate oxidase. The steady-state lactate electrooxidation current, at 2mM lactate concentration and at 22 deg C, was 4OO nA. The 5O plus or minus 10 microA/sq cm current density and the 2OmA/sq cm,/M sensitivity decreased only by 5% upon increasing the partial pressure of oxygen from 0.0 to 0.2 atm. The electrode retains its sensitivity after dry storage at 4 deg C for 4 months in air but loses at 37 deg C half of its sensitivity in 7 hours through polymer desorption when operated at 0.4V (SCE).


Descriptors :   *PROBES , *CARBON FIBERS , *ELECTRODES , *LACTATES , STEADY STATE , DENSITY , POLYMERS , SENSITIVITY , AMINES , ETHERS , ELECTROLYTIC CELLS , VINYL RADICALS , TUBES , PYRIDINES , ALDEHYDES , ETHYL RADICALS , DESORPTION , EPOXY COMPOUNDS , STORAGE , AZOLES , POLYIMIDE RESINS , OXIDOREDUCTASES , PARTIAL PRESSURE , ANODES , IMIDAZOLES , PRESSURE , OXIDATION , OXYGEN , CURRENT DENSITY , ENZYMES , FIBERS , DIAMETERS


Subject Categories : Biochemistry
      Organic Chemistry
      Refractory Fibers


Distribution Statement : APPROVED FOR PUBLIC RELEASE