Accession Number : ADA260574
Title : Cellular and Tissue Injury During Nonfreezing Cold Injury and Frostbite.
Descriptive Note : Tri-annual rept. no. 6, Sep-Nov 92.
Corporate Author : CRYOLIFE INC MARIETTA GA
Report Date : 08 Dec 1992
Pagination or Media Count : 6
Abstract : Metabolic perturbation and enzyme structure-function studies. We have now almost completed our investigation of the structure-function relationships that are thought to be involved with regulating activity of phosphofructokinase (PFK) in vivo. Specifically, we set out to study how alterations in quaternary structure account for enzyme inhibition in red blood cells under cold, acidotic conditions. Prior in vitro studies have shown that phosphofructokinase from erythrocytes (and several other mammalian cells and tissues) dissociates from active tetrameres into inactive dimers and monomers at low Ph. In addition, we have previously demonstrated that this reversible phenomenon accounts for the inhibition of PFK activity in vivo in ischemic myocardium.
Descriptors : *TISSUES(BIOLOGY) , *WOUNDS AND INJURIES , *METABOLIC DISEASES , *FROSTBITE , IN VITRO ANALYSIS , HYPOTHESES , IN VIVO ANALYSIS , CELLS(BIOLOGY) , AMINO ACIDS , COLD TOLERANCE , BLOOD CELLS
Subject Categories : Anatomy and Physiology
Medicine and Medical Research
Distribution Statement : APPROVED FOR PUBLIC RELEASE