Accession Number : ADA260180
Title : Diagnosis of Natural and Induced Diseases of Military Importance. Phase 1
Descriptive Note : Final rept.
Corporate Author : ADEZA BIOMEDICAL SUNNYVALE CA
Personal Author(s) : Charlton, David E
Report Date : 05 Oct 1990
Pagination or Media Count : 15
Abstract : The purpose of this research project was to demonstrate the feasibility of the Optical Biossay (OBA) system for the quantitation of circulating serum lgG and lgM antibodies against Hantaan virus. Reference EUSA assays were set up to measure lgG and lgM antibodies to Hantaan virus. A number of different viral culture extracts and viral constructs were evaluated for use in developing the Optical Bioassay. The viral culture extract was used in developing the lgG assay, and a viral construct was used in developing the lgM assay. The lgG assay was formatted as a 2 step 1 0 minute test with the viral lysate attached to the OBA test chip and monoclonal anti-human lgG coupled to colloidal gold as the signal The lgM assay was formatted as an lgM capture assay with anti-IgM immobilized on the OBA test chip and the Hantaan construct coupled to colloidal gold as the signal enhancer. This required 2 steps and 1 hour. Optimization of the assays should reduce the test to 10 minutes in a 1 step format, but would require additional time to develop more purified viral reagents and optimize assay parameters. A prototype reader was developed during the program, and used to read the assay results. Both test formats were evaluated using 7 positive and 6 negative serum samples. In all cases the OBA results correlated 100% with the ELISA results.
Descriptors : *DIAGNOSIS(MEDICINE) , *MILITARY MEDICINE , *HANTAAN VIRUS , TEST AND EVALUATION , QUANTITATIVE ANALYSIS , OPTIMIZATION , ANTIBODIES , BLOOD SERUM , BIOASSAY , CULTURES(BIOLOGY) , GOLD , SIGNALS , HUMANS , PARAMETERS , PROTOTYPES
Subject Categories : Medicine and Medical Research
Distribution Statement : APPROVED FOR PUBLIC RELEASE