Accession Number : ADA259908
Title : Detection of Picogram Quantities of Botulinus Toxin-B Using the Light Addressable Potentiometric Sensor.
Descriptive Note : Final rept. Sep 91-Jan 92,
Corporate Author : TEXAS UNIV AT SAN ANTONIO
Personal Author(s) : Chambers, James P ; Valdes, James J
Report Date : Dec 1992
Pagination or Media Count : 14
Abstract : Detection of picogram quantities of Botulinus toxin-B (BoTX), using Protein A purified biotin- and fluorescein-labelled antibodies, is described in this report. All assays were performed on the Light Addressable Potentiometric Sensor. Results indicate a near linear dose response curve for increasing the amounts of BoTX (10-1,000 pg). The presence of 1-30 ng of toxin resulted in a dose response curve that yielded high output signals (4,000 microV/s) at saturation (approximately 25 ng). When challenged with nine different venoms, the biosensor indicated very low output signals (15% control), which indicated minimal biosensor nonspecificity.... Receptors, Biosensors, Toxins.
Descriptors : *PROTEINS , *TOXINS AND ANTITOXINS , CONTROL , DETECTION , LIGHT , SIGNALS , RESPONSE(BIOLOGY) , EXOTOXINS , VITAMIN B COMPLEX , RECEPTOR SITES(PHYSIOLOGY) , VENOMS , ANTIBODIES , SATURATION , REPORTS , QUANTITY
Subject Categories : Biochemistry
Distribution Statement : APPROVED FOR PUBLIC RELEASE