Accession Number : ADA259835
Title : In Vitro System for the Assessment of the Potential Antifertility Affect of Chemicals.
Descriptive Note : Final rept. Feb 89-Sep 90,
Corporate Author : CHEMICAL RESEARCH DEVELOPMENT AND ENGINEERING CENTER ABERDEEN PROVING GROUND MD
Personal Author(s) : Young, R J ; Iturralde, T ; Starke, W C
Report Date : Jul 1992
Pagination or Media Count : 17
Abstract : The medium and culture conditions that support rabbit sperm cell motility development of hyperactive motion were determined. Sperm motility was excellent when cultured in defined medium (DM) supplemented with heat inactivated rabbit, calf, or horse serum; but cell viability was poor. Cell viability was improved in DM alone or in Biggers, Whittingham, and Whitten medium but was best when incubated in Toyoda and Chang or T6 mediums. Hyperactive motion developed in the four media when incubation was carried out at 37 deg C in 95% air and 5% CO2 . A preincubation step was not necessary. Sperm cell viability and hyperactive motility development was rabbit specific... . Medium, Rabbit, Preincubation, Serum, Hyperactive motion, Gas mixture, Sperm cells, Viability.
Descriptors : *CHEMICALS , AIR , CELLS , MOTION , IN VITRO ANALYSIS , GASES , MIXTURES , VIABILITY , ADVERSE CONDITIONS , INCUBATION , MEDIA , HEAT , CULTURE , TOXICOLOGY , RABBITS , REPRODUCTION(PHYSIOLOGY) , HORSES , FERTILITY
Subject Categories : Biochemistry
Anatomy and Physiology
Distribution Statement : APPROVED FOR PUBLIC RELEASE