Accession Number : ADA259832
Title : Post-Fusion Membrane Reorganization.
Descriptive Note : Final rept. 28 Oct 91-31 Jan 93,
Corporate Author : MARYLAND UNIV BALTIMORE SCHOOL OF MEDICINE
Personal Author(s) : Sowers, Arthur E
Report Date : 27 Jan 1993
Pagination or Media Count : 9
Abstract : We have previously shown by thin section electron microscopy (BJ 60: 1037 in a contact zone induced between erythrocyte ghosts by dielectrophoresis, the use of an electrofusion protocol converts the contact zone into a fusion zone (FZ) containing from 1-225 fusion pores per micrometer squared. Also, after creation, both the FZ stability, and the time-dependent diameter expansion rates of the fusion zone are strongly dependent on an intact spectrin network. In the present project, and using computer-assisted analysis on video-recorded phase optics images of fusion zone diameters, we have found that the FZ diameter vs. time kinetics has two, or three, distinct phases (I-III) in erythrocyte ghosts with an intact or heat-disrupted (42 oC, 20 min), respectively, spectrin network. These phases, revealed by FZ diameter vs. time measurements under some combinations of various electric pulse parameters, temperatures (4-33 deg C) during measurements, heat treatment temperatures (39-50 deg C), and dielectrophoretic force (E sub ac) =3.25, 4.25, and 5.25 V/mm), and under certain conditions, were remarkably independent from one another, yet the durations of Phase I (1.0-1.2 sec) and Phase II (4.0 sec) were remarkably invariant regardless of the variable studied. This suggested the existence of a complex but dissectable interplay of biomechanical factors....Membranes, Cells, Electrofusion, Electroporation, Red cells, Erythrocytes.
Descriptors : *MEMBRANES , *ERYTHROCYTES , CONTROL , DIAMETERS , MEASUREMENT , OPTICS , TEMPERATURE , STABILITY , MODELS , NETWORKS , COMPUTERS , PARAMETERS , CELLS , BIOCHEMISTRY , DIELECTRICS , RATES , REGIONS , PHASE , HEAT TREATMENT , DISSECTION , AGGLUTININS , MICROMETERS , WHEAT , GLYCEROLS , ETHANOLS , ELECTROPHORESIS , PH FACTOR , BIOMECHANICS , VIRUSES , HEAT , ELECTRIC CURRENT , AMIDES , KINETICS , EXPANSION , MICROSCOPY , TIME , VARIABLES , ELECTRON MICROSCOPY , CROSSLINKING(CHEMISTRY) , ELECTRONS , PULSES , IMAGES
Subject Categories : Biochemistry
Anatomy and Physiology
Distribution Statement : APPROVED FOR PUBLIC RELEASE