Accession Number : ADA259792


Title :   Simultaneous Detection of Multiple Disease States.


Descriptive Note : Final rept. 15 Aug 86-15 Feb 90,


Corporate Author : BIOSTAR MEDICAL PRODUCTS INC BOULDER CO


Personal Author(s) : Lopez, Luis


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a259792.pdf


Report Date : 14 Feb 1990


Pagination or Media Count : 26


Abstract : The objectives for this project were to demonstrate the utility of BioStar Optical ImmunoAssay (OIA) tm for the detection of infectious agents in insect populations using malaria and Lyme antigens as models. Also to demonstrate 'DIA applicability for the diagnosis of host response to malarial infections. To meet the latter objective a recombinant antigen specific to P. falciparum was immobilized to a propertiary solid support, which provided the desired optical properties, and samples assayed for the presence of antibody to this antigen. Specificity of this antibody response was clearly demonstrated. For the detection of Malaria or Lyme antigens, the appropriate monoclonal antibodies were immobilized to the optical solid support. Samples were examined for the presence of antigen in heat-inactivated organisms or infected carriers (mosquitos or ticks). Developed extraction protocols allowed uninfected organisms to be assayed without the generation of a signal. OIA's flexibility allows any number of analytes to be assayed in a panel forma And due to its simplicity, OIA has been demonstrated to be generally applicable to a wide range of testing environments. Since unstable reagents are not generally employed there are no special storage requirements for field tests.... Interference signal, Immunoassay.


Descriptors :   *INFECTIOUS DISEASES , *IMMUNOASSAY , *DISEASE VECTORS , TEST AND EVALUATION , REQUIREMENTS , OPTICAL PROPERTIES , ENVIRONMENTS , DETECTION , MODELS , FIELD TESTS , POPULATION , DEOXYRIBONUCLEIC ACIDS , RESPONSE , SIGNALS , INTERFERENCE , EXTRACTION , GENES , STORAGE , MONOCLONAL ANTIBODIES , MALARIA , VIROLOGY , ANTIGENS , TICKS , HEAT , PLASMODIUM FALCIPARUM


Subject Categories : Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE