Accession Number : ADA259749


Title :   Possible Involvement of Endogenous Beta-Endorphin in the Pathophysiological Mechanisms of Pichinde Virus-Infected Guinea Pigs


Corporate Author : ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD


Personal Author(s) : Guo, Z M ; Liu, C T ; Peters, C J


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a259749.pdf


Report Date : Jan 1992


Pagination or Media Count : 6


Abstract : Previously, we demonstrated that naloxone, an opiate antagonist, prolonged survival of strain 13 guinea pigs infected with Pichinde virus. Thus, endogenous opiates may be involved in the pathogenesis of this viral disease. To determine whether endogenous opiate levels were affected by Pichinde viral infection, Beta-endorphin concentrations in plasma and cerebrospinal fluid (CSF) of normal and infected strain 13 guinea pigs were measured by radioimmunoassay. Cerebrospinal fluid Beta-endorphin concentrations were 78.0 +/- 13.2 pg/mi on postinoculation day (PID) 7, 59.0 +/- 5.6 pg/mi on PID 12, and 58.8 +/- 5.4 pg/mi on PID 14. These values were significantly higher than baseline levels of CSF Beta-endorphin: 30.8 +/- 1.9 pg/mi. Plasma Beta-endorphin concentrations of infected animals increased significantly to 202.1 +/- 17.9 pg/ml on PID 7 and to 154.2 +/- 21.4 pg/mi on PID 12 from a mean baseline value of 84.2 +/- 13.1 pg/mi. After a primer intravenous injection of Beta-endorphin (10, 15, or 30 jig/kg), followed by constant infusion of Beta-endorphin (15, 45, or 90 microg/kg(dot)hr) to control noninfected guinea pigs, heart rate (except with the lowest dose) and mean blood pressure decreased markedly. Under these experimental conditions, concentrations of plasma and CSF Beta-endorphin increased simultaneously with different magnitude. Because both Pichinde viral infection and Beta-endorphin administration produced a similar trend of cardiovascular disturbances, leading to hypotension and bradycardia, increased concentrations of plasma and CSF Beta-endorphin may play a partial role in the pathophysiological mechanisms of Pichinde virus infection.


Descriptors :   *PATHOPHYSIOLOGY , *GUINEA PIGS , *ENDORPHINS , CONTROL , INJECTION , MANAGEMENT , BRAIN , PITUITARY GLAND , PRIMERS , HEART RATE , PHARMACOLOGICAL ANTAGONISTS , CEREBROSPINAL FLUID , OPIUM ALKALOIDS , RADIOIMMUNOASSAY , INFUSIONS , BRADYCARDIA , HEMORRHAGE , LABORATORY ANIMALS , BLOOD PRESSURE , BLOOD PLASMA , HYPOTENSION , PATHOGENESIS , CONSTANTS , STRAINS(BIOLOGY) , FLUIDS , MEAN , INFECTIOUS DISEASES , VIRUS DISEASES


Subject Categories : Medicine and Medical Research
      Microbiology


Distribution Statement : APPROVED FOR PUBLIC RELEASE