Accession Number : ADA259484


Title :   A Medical Research and Evaluation (MREF) Facility and Studies Supporting the Medical Chemical Defense Program


Descriptive Note : Final rept. Sep 1989-Nov 1992


Corporate Author : BATTELLE MEMORIAL INST COLUMBUS OH


Personal Author(s) : Hobson, David W ; Miller, Larry S ; Bhullar, Balwant S ; Moore, Victor S


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a259484.pdf


Report Date : Nov 1992


Pagination or Media Count : 69


Abstract : The purpose of this task was to produce and characterize rabbit anti- atropine antisera for future analytical studies, and to establish and validate the Walter Reed Army Institute of Research (WRAIR) atropine radioimmunoassay (RIA) at Battelle's Columbus Laboratories. For Phase I of this task, an atropine- BSA conjugate was prepared and characterized. This conjugate had an atropine:BSA molar ratio of approximately 32:1, and was used to immunize 12 atropinase-free New Zealand White rabbits for periods of 13 or 21 months using two separate schedules. Antisera were collected and titrated using the RIA procedure. Two rabbits having the highest titer were identified, and their sera were pooled for RIA development. Although the titers of these sera were 10-20- fold lower than antisera from Dr. Smallridge at WRAIR, the RIA characteristics were similar. Using a 1:600 dilution, standard curves were generated with consistent detection limits of 3.0 ng/ml and response ranges extending to 20 ng/ml. A representative standard curve had a slope of 0.984, a y-intercept of 5. 975, an ED,, of 432.7 pg/50 microliters, and a bound cpm/total cpm (B/T) value of 0.32.


Descriptors :   *ATROPINE , *RADIOIMMUNOASSAY , DETECTION , ANTIBODIES , PHARMACOKINETICS , MEDICAL RESEARCH , DILUTION


Subject Categories : Biochemistry
      Pharmacology


Distribution Statement : APPROVED FOR PUBLIC RELEASE