Accession Number : ADA259465


Title :   Dengue-1 Virus Envelope Glycoprotein Gene Expressed in Recombinant Baculovirus Elicits Virus-Neutralizing Antibody in Mice and Protects them from Virus Challenge


Descriptive Note : Final rept.,


Corporate Author : WALTER REED ARMY INST OF RESEARCH WASHINGTON DC


Personal Author(s) : Putnak, Robert ; Feighny, Robert ; Burrous, Jeanne ; Cochran, Mark ; Hackett, Craig


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a259465.pdf


Report Date : Jan 1991


Pagination or Media Count : 10


Abstract : In an effort explore the feasibility of a baculovirus recombinant dengue-1 (DEN 1) virus vaccine, a portion of the envelope (E) glycoprotein gene from DEN 1 virus was cloned and expressed in baculovirus (autographa californica nuclear polyhedrosis virus, AcNPV). The recombinant construct contains 107 nucleotides from the 3' terminus of the M gene which encodes a hydrophobic signal peptide and extends through the first 1245 nucleotides of E, terminating 243 nucleotides before the 3' terminus of E. When the recombinant was grown in Spodoptera frugiperdal cells, about 1 mg of E antigen was made per 10


Descriptors :   *GLYCOPROTEINS , *ANTIBODIES , *MONOCLONAL ANTIBODIES , *VACCINES , *GENES , *DENGUE , PRODUCTION , PEPTIDES , RESPONSE , LETHALITY , HEAT , VIROLOGY , IMMUNOGENS , NUCLEOTIDES , AMINO ACIDS , ANTIGENS , CELLS(BIOLOGY) , VIRUS DISEASES , INFECTIOUS DISEASES , MICE , CLONES , STRAINS(BIOLOGY) , SIGNALS , PROTEINS , DISEASES


Subject Categories : Medicine and Medical Research
      Microbiology
      Pharmacology


Distribution Statement : APPROVED FOR PUBLIC RELEASE