Accession Number : ADA259402


Title :   Electrode Probes for the Rapid Assay of Seafood Toxicants. Phase 1


Descriptive Note : Final rept. 15 Aug 1989-15 Mar 1990


Corporate Author : UNIVERSAL SENSORS METAIRIE LA


Personal Author(s) : Ramsay, Graham ; Lubrano, G J ; Nordyke, L L ; Guilbault, G G


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a259402.pdf


Report Date : 01 Mar 1990


Pagination or Media Count : 29


Abstract : An enzyme-limited immunosorbent-assays (ELISA) with electrochemical detection was developed for the seafood toxin okadaic acid (diarrhetic shellfish poison). At low ppb concentrations, competitive heterogeneous immunoassays were performed in which soluble sample antigen (50 pl) competed with immobilized antigen for soluble antibody (50 pl). Excess sample and antibody were removed by washing and a secondary antibody labeled with alkaline phosphatase was added. The enzyme label hydrolyzed phenyl phosphate to yield phenol which was oxidized at +870 mV vs. Ag/AgCl. The dynamic response was inversely proportional to soluble antigen concentration.... Electrochemical immunoassay, seafood toxins, okadaic acid, saxitoxin.


Descriptors :   *TOXICITY , *ELECTRODES , *IMMUNOASSAY , *ELECTRON PROBES , *SEAFOOD , DETECTION , ENZYMES , DYNAMIC RESPONSE , YIELD , ANTIBODIES , LETHALITY , RICIN , POISONS , ANTIGENS , PHENOLS , TOXINS AND ANTITOXINS , LABELS , PHOSPHATES , SHELLFISH , PHOSPHATASES


Subject Categories : Medicine and Medical Research
      Toxicology
      Pharmacology


Distribution Statement : APPROVED FOR PUBLIC RELEASE