Accession Number : ADA259161


Title :   Specific Detection of Campylobacter Jejuni and Campylobacter Coli by Using Polymerase Chain Reaction


Descriptive Note : Journal article,


Corporate Author : NAVAL MEDICAL RESEARCH INST BETHESDA MD


Personal Author(s) : Oyofo, Buhari A ; Thornton, Scott A ; Burr, Donald H ; Trust, Trevor J ; Pavlovskis, Olgerts R


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a259161.pdf


Report Date : Oct 1992


Pagination or Media Count : 8


Abstract : Development of a routine detection assay for Campylobacter jejuni and Campylobacter coli in clinical specimens was undertaken by using the polymerase chain reaction(PCR). An oligonucleotide primer pair from a conserved 5' region of the flaA gene of C. coli VC167 was used to amplify a 450-bp region by PCR. The primer pair specifically detected 4 strains of C.coli and 47 strains C. jejuni; but it did not detect strains of Campylobacter fetus, Campylobacter lari, Campylobacter upsaliensis, Campylobacter cryaerophila, Campylobacter butzleri, Campylobacter hyointestinalis, Wolinella recta, Helicobacter pylori, Escherichia coli, Shigella spp., Salmonella spp., Bibrio cholerae, Citrobacter freundii, or Aeromonas spp. By using a non radioactively labeled probe internal to the PCR product, the assay could detect as little as 0.0062 pg of purified C. coli in DNA, or the equivalent of four bacteria. In stools steeded with C. coli cells, the probe could detect between 30 and 60 bacteria per PCR assay. the assay was also successfully used to detect C. coli in rectal swab specimens form experimentally infected rabbits and C.jejuni in human stool samples.


Descriptors :   *STRAINS(BIOLOGY) , *ANTIGENS , *CHAIN REACTIONS , BACTERIA , DEOXYRIBONUCLEIC ACIDS , BACTERIOLOGY , ESCHERICHIA COLI , GENES , DIAGNOSIS(MEDICINE) , DIARRHEA


Subject Categories : Medicine and Medical Research
      Microbiology


Distribution Statement : APPROVED FOR PUBLIC RELEASE