Accession Number : ADA258657
Title : Evaluation of Dried Storage of Platelets for Transfusion: Physiologic Integrity and Hemostatic Functionality.
Descriptive Note : Triannual rept. no. 2, 1 Jun-30 Nov 92,
Corporate Author : EAST CAROLINA UNIV SCHOOL OF MEDICINE GREENVILLE NC
Personal Author(s) : Bode, Arthur P
Report Date : 02 Dec 1992
Pagination or Media Count : 8
Abstract : All equipment purchases have been completed. The new Virtus lyophilizer was installed at ECU in August and quickly pressed into service; its first run was on September 15. Now the lyophilizer at the Chapel Hill worksite can be dedicated to bulk animal cell preparations for in vivo testing, while the ECU lyophilizer will be used to optimize preparation technique with human or animal cells. The results of several optimization runs are noted below. The study of metabolic activity of rehydrated platelets was originally proposed as an intramural collaboration at ECU with the Department of Radiation Oncology Biology. Due to a change in faculty, this plan was abandoned and a new arrangement has been made with collaborators at the Mid-Atlantic Regional Headquarters of the American Red Cross to carry out these studies. Stein Holme, Ph.D., established investigator of the American Red Cross and director of the blood research laboratory in Norfolk, VA, will perform oxygen consumption measurements and C(14) adenine radioisotope tracer studies on lyophilized platelet preparations made at ECU or UNC to determine the integrity of metabolic pathways in reconstituted cells. We are optimistic with this new approach because of the past productive collaboration with the Red Cross laboratory during the conduct of our first ONR grant project (N00014-89-J-1712). A subcontract document was signed by both parties September 2, and several samples are now undergoing analysis.
Descriptors : *BLOOD PLATELETS , *BLOOD STORAGE , FUNCTIONAL ANALYSIS , THERMAL SHOCK , LABORATORY ANIMALS , BLOOD TRANSFUSION
Subject Categories : Biochemistry
Medicine and Medical Research
Distribution Statement : APPROVED FOR PUBLIC RELEASE