Accession Number : ADA258576
Title : An Assessment of a Rapid Microwave Thawing Procedure for Frozen Red Blood Cells.
Descriptive Note : Master's thesis,
Corporate Author : AIR FORCE INST OF TECH WRIGHT-PATTERSON AFB OH
Personal Author(s) : Davis, Dianne C
Report Date : Aug 1992
Pagination or Media Count : 85
Abstract : Paired units of frozen red blood cells (RBC) were thawed in a conventional 42 deg C waterbath and a microwave oven in order to determine the suitability of the microwave method for thawing RBC. A structurally modified microwave oven equipped with rotating mixing arms and temperature sensors was used. Measurements of freeze-thaw-wash recovery, supernatant potassium, osmotic fragility, adenosine 5'-triphosphate and 2,3-diphosphoglycerate revealed no significant differences (p greater than or = 0.05) between the thawing methods. Microwave-thawed RBC units had significantly increased levels of supernatant hemoglobin and white blood cells. The elevated white blood cell counts artificially reflected microaggregate formation. Overall, the microwave-thawed RBC were similar to the waterbath-thawed control RBC indicating that the functional and biologic integrity of the blood was not altered by the electromagnetic radiation. Thawing time averaged one-fifth as long using the microwave oven making this thawing method an attractive, time-saving alternative to the currently used waterbath method.
Descriptors : *MICROWAVES , *FREEZING , *ERYTHROCYTES , CONTROL , MEASUREMENT , TEMPERATURE , LOW TEMPERATURE , MIXING , STORAGE , HEMOGLOBIN , ADENOSINE , BLOOD CELLS , OVENS , FRAGILITY , MICROWAVE OVENS , LEUKOCYTES , BLOOD , POTASSIUM , SAVINGS , CONTAMINATION , THAWING , ELECTROMAGNETIC RADIATION , TIME , RECOVERY , BACTERIA , CELLS
Subject Categories : Medical Facilities, Equipment and Supplies
Radiofrequency Wave Propagation
Distribution Statement : APPROVED FOR PUBLIC RELEASE