Accession Number : ADA256166


Title :   Stress, Chemical Defense Agents and Cholinergic Receptors


Descriptive Note : Final rept. 1 Nov 1987-30 Jun 1991


Corporate Author : TEXAS COLL OF OSTEOPATHIC MEDICINE FORTWORTH DEPT OF PHARMACOLOGY


Personal Author(s) : Lane, John D


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a256166.pdf


Report Date : 31 Jul 1991


Pagination or Media Count : 64


Abstract : The goal of this project is to assess the effects of exposure to a chemical defense agent (XGD; soman) on anxiety and stress, by using rat models of anxiety (conditioned emotional response, CER) and unconditioned non-specific stress. To test for changes in anxiety, extinction from CER was measured, based on the hypothesis that increased anxiety produced by environmental exposures would prolong extinction. The specific experiments determined the plasticity of muscarinic cholinergic binding-sites in the central nervous system. The effects of acute exposure to doses of soman on lethality and well-characterized behaviors were examined, and found to be consistent with previous reports. The binding of radiolabelled cholinergic (ACh) ligands to brain tissue was studied in vitro. The major findings are that CER produces increases in acetycholine turnover in brain areas involved in anxiety, and that primarily post-synaptic M1 ACh receptors decrease. These neurochemical phenomena are directly correlated with several behaviors, including acquisition and extinction of CER and non- specific stress. If soman exposure increased anxiety under any conditions, this should be reflected in increased time of CER extinction and changes in receptors; the behavioral response was not observed, so receptor function was not assessed. Therefore, the effect of soman exposure on anxiety is not likely to be specific to the cholinergic system. Anxiety, Acetylcholine, CD Agents, Acetylcholinesterase, Inhibitors, Brain receptors, RA1.


Descriptors :   *CENTRAL NERVOUS SYSTEM , *CHEMICAL WARFARE AGENTS , *CHOLINERGIC NERVES , TEST AND EVALUATION , ACQUISITION , BRAIN , MODELS , RATS , CHEMICALS , SITES , TIME , RESPONSE , LIGANDS , NERVOUS SYSTEM , PLASTIC PROPERTIES , LETHALITY , EXPOSURE(PHYSIOLOGY) , INHIBITORS , ACETYLCHOLINESTERASE , ACETYLCHOLINE , MUSCARINE , ANXIETY


Subject Categories : Anatomy and Physiology
      Toxicology
      Chemical, Biological and Radiological Warfare


Distribution Statement : APPROVED FOR PUBLIC RELEASE