Accession Number : ADA255607


Title :   Autoradiographic Distribution and Applied Pharmacological Characteristics of Dextromethorphan and Related Antitissue/Anticonvulsant Drugs and Novel Analogs.


Descriptive Note : Annual rept. 15 Jul 1991-14 Jul 1992,


Corporate Author : LONDON UNIV (UNITED KINGDOM) DEPT OF PHARMACOLOGY


Personal Author(s) : Bowery, Norman G


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a255607.pdf


Report Date : 01 Aug 1992


Pagination or Media Count : 12


Abstract : Binding of dextromethorphan and its analogues to the dextromethorphan binding site and to the PCP and glycine binding sites associated with the N- methyl-D-aspartate receptor-channel complex was investigated using 3H-DM, H-TCP and 3H-glycine as radioligands. The experiments were carried out in 1m1 tubes (miniblocks), in a final incubation volume of 0.5m1. All reagents were diluted and dispensed using a TECAN and filtering was done using a Brandel M-48R. Samples were counted in 5m1 of Esoscint A. after an extraction period of at least 6 hours, using a Beckman LS1701 liquid scintillation counter. All compounds were dissolved in distilled water except compound 3 which was dissolved in DMSO/lactic acid and diluted with distilled water. Standards were obtained from commercial sources except dichlorokynurenic acid (DCK) which was a gift from Pfizer Central Research, Sandwich, Kent. Drugs, RAIII, Anticonvulsants, Antitissues, Dextromethorphan, Autoradiography, Pharmacokinetics.


Descriptors :   *PHARMACOKINETICS , *ANTICONVULSANTS , VOLUME , TISSUES(BIOLOGY) , BRAIN , SITES , BINDERS , ACIDS , SCINTILLATION , GLYCINE , COUNTERS , LACTIC ACID , TISSUE CULTURE , DISTILLED WATER , AUTORADIOGRAPHY , SCINTILLATION COUNTERS , FILTRATION , CHANNELS , DRUGS , STANDARDS , EXTRACTION , INCUBATION


Subject Categories : Anatomy and Physiology
      Pharmacology


Distribution Statement : APPROVED FOR PUBLIC RELEASE