Accession Number : ADA255567


Title :   The Synthesis of Chemiluminescent Flavoabzymes and Their Use in Metal Ion Detection.


Descriptive Note : Final rept. 15 Mar 1990-15 Sep 1992,


Corporate Author : CALIFORNIA UNIV SANTA BARBARA DEPT OF CHEMISTRY


Personal Author(s) : Bruice, Thomas C


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a255567.pdf


Report Date : 02 Sep 1992


Pagination or Media Count : 9


Abstract : The ultimate goal of this research is to develop chemiluminescent (CL) abzymes and a technology for their use in metal ion detection. Four objectives must be reached in sequential order:.1st, a molecule (hapten) which resembles in space requirements an intermediate in a CL reaction must be synthesized; 2nd, a hapten protein conjugate (antigen) must be prepared and used to obtain monoclonal antibodies (Mab's); 3rd, one or more of the Mab's must catalyze the given CL reaction; and .4th , the hapten must be-modifiable such that the CL Mab's become specific complexers of metal ions. Accomplishment of the 1st objective would provide the first Mab mimic of bioluminescence. Attempts to synthesize a hapten to generate Cl flavoabzymes will be discussed as will progress in the synthesis of a hapten to be used to prepare abzymes to catalyze the CL reaction of H202 with oxalate esters Monoclonal antibodies (Mab's); Abzymes; hapten; antigen; chemiluminescence (CL); bioluminescence.


Descriptors :   *IONS , *METALS , *DETECTION , *SYNTHESIS , *CHEMILUMINESCENCE , *BIOLUMINESCENCE , REQUIREMENTS , MOLECULES , PROTEINS , ANTIBODIES , MONOCLONAL ANTIBODIES , ESTERS , ANTIGENS , OXALATES , HYDROGEN PEROXIDE


Subject Categories : Biochemistry
      Organic Chemistry
      Radiation and Nuclear Chemistry
      Optics


Distribution Statement : APPROVED FOR PUBLIC RELEASE