Accession Number : ADA254744


Title :   Studies of Infection and Dissemination of Rift Valley Fever Virus in Mosquitoes.


Descriptive Note : Final rept. 15 May 89-14 Sep 91,


Corporate Author : OHIO UNIV ATHENS


Personal Author(s) : Romoser, William S


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a254744.pdf


Report Date : 15 Oct 1991


Pagination or Media Count : 182


Abstract : During the past two years, we have carried out the following studies:(l) Ultrastructural study of Rift Valley fever (RVF) virions in the cardia. (2) Immunocytochemical studies of the salivary glands and other tissues in Anopheles sp. which had been shown to be non-transmitters when intrathoracically-infected as adults, but transmitters when infected as immatures. (3) Immunocytochemical study of Ockelbo virus in Culex pipiens. (4) Ultrastructural examination of various tissues for RVF virus in hemocoelically- infected Cx. pipiens. (5) Development of an immunogold procedure for in situ labelling of RVF virions in electron microscopic preps. (6) Worked toward the identification and isolation of the mosquito cell surface receptor molecule for RVF virus. (7) Developed and presented a pre-meeting workshop, Mosquito Histology, Ultrastructure, and Methods for In situ Localization of Viruses, held in conjunction with the annual meeting of the American Society of Tropical Medicine and Hygiene. (8) Made progress in the development of a monograph on mosquito histology and ultrastructure. Rift Valley fever, mosquitos, infection, dissemination, mosquito tissue tropisms, infection and escape barriers , electron microscopy, immunogold-labelling; immunocytochemistry, cell surface receptor, Ockelbo virus,


Descriptors :   *INFECTIOUS DISEASES , *RIFT VALLEY FEVER , *VIRUSES , *MOSQUITO BORNE DISEASES , SOCIETIES , MOLECULES , CELLS , ISOLATION , MICROSCOPY , ADULTS , ANOPHELES , SALIVARY GLANDS , IMMUNOCHEMISTRY , CULICIDAE , GLANDS , HYGIENE , HISTOLOGY , FEVERS , WORKSHOPS , TROPICAL MEDICINE , CULEX , VALLEYS , ELECTRON MICROSCOPY , IDENTIFICATION , SURFACES , MEDICINE , ELECTRONS , TRANSMITTERS


Subject Categories : Biology
      Ecology


Distribution Statement : APPROVED FOR PUBLIC RELEASE