Accession Number : ADA254742


Title :   Molecular Recognition of Alpha-Neurotoxins


Descriptive Note : Final rept. 15 Mar 1989-15 Aug 1992


Corporate Author : BAYLOR COLL OF MEDICINE HOUSTON TX DEPT OF BIOCHEMISTRY


Personal Author(s) : Atassi, M Z


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a254742.pdf


Report Date : 15 Aug 1992


Pagination or Media Count : 57


Abstract : Peptides representing all the loops and surface regions of a- bungarotoxin were synthesized. Control peptide analogs were also prepared in which these sequences were randomized. The peptides were used to map the profiles of the antibodies and T cells obtained after BgTX immunization. Also, the abilities of anti-peptide antibodies and T cells to recognize the immunizing peptide and BgTX were determined. Immunization of mice with the individual peptides afforded significant protection against BgTX challenge. The most protective peptides, Ll, L2 and C-tail, allowed the mice to survive three times the LD50 of the controls (i.e., Protective Index = 3). Immunization with an equimolar mixture of the three peptides gave better protection (P.I. = 4.6), while an ovalbumin conjugate carrying these three peptides simultaneously afforded enormous protection (P.I. = 18.1) which was much higher than the protection by BgTX immunization (P.I. = 9.7). It is concluded that the multi- peptide conjugate should serve as an excellent vaccine against BgTX. The work is being prepared for a patent application. RA 1; Synthetic peptides; Alpha- neurotoxins.


Descriptors :   *PEPTIDES , *IMMUNIZATION , *VACCINES , *NEUROTOXINS , CONTROL , PATENT APPLICATIONS , SEQUENCES , MIXTURES , PROFILES , PROTECTION , ANTIBODIES , MAPS , ANALOGS , MOLECULAR STRUCTURE , MICE , CELLS(BIOLOGY) , PATENTS


Subject Categories : Biochemistry
      Pharmacology


Distribution Statement : APPROVED FOR PUBLIC RELEASE