Accession Number : ADA254605


Title :   Biodegradation of Polychlorinated Methanes in Methanogenic Systems


Descriptive Note : Annual rept. 1 Dec 1988-1 Mar 1989,


Corporate Author : ILLINOIS UNIV AT URBANA DEPT OF CIVIL ENGINEERING


Personal Author(s) : Freedman, David L


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a254605.pdf


Report Date : Jan 1991


Pagination or Media Count : 110


Abstract : This research investigated biodegradation of several halogenated methanes under methanogenic conditions. Biodegradation of dichloromethane to C02 and acetic acid (both environmentally acceptable products) was demonstrated in a fixed-film reactor, operated at 20 deg C, a residence time as low as 0.25 day, and an influent concentration of 91 micro M. The biodegradability of chloroform was examined in a dichloromethane-degrading enrichment culture. Sustained consumption of chloroform (approximately 8.5 micron M) was achieved only when vitamin B(12) was also added. Initially, equimolar amounts of chloroform and B(12) were added; when the amount of B(12) added was gradually decreased to zero, chloroform degradation continued at the same rapid rate. Biodegradation of 14C chloroform yielded approximately 77% C02, 10% carbon monoxide, 7% soluble compounds (about 15% of which consisted of acetate), and 2% nonsoluble compounds; no 14CH4 was formed. Brominated methanes (dibromomethane, bromochloromethane, and bromomethane) degraded much more slowly' if at all, than dichloromethane, they inhibited methanogenesis and dichloromethane degradation. Sustained degradation of chloromethane was demonstrated for an extended period without the need for an electron donor. Preliminary results suggest chloromethane serves as an electron donor under methanogenic conditions, just as dichloromethane does. Biotransformation of carbon tetrachloride was also demonstrated in the dichloromethane-degrading enrichment culture.


Descriptors :   *BIODETERIORATION , *METHANES , DEGRADATION , FILMS , RATES , CARBON , TIME , ELECTRONS , SOLVENTS , ELECTRON DONORS , CONSUMPTION , CHLOROMETHANES , CARBON TETRACHLORIDE , ACETIC ACID , MONOXIDES , CARBON MONOXIDE , CULTURE , ENRICHMENT , ACETATES , ACIDS , CHLOROFORM , VITAMIN B COMPLEX


Subject Categories : Physical Chemistry


Distribution Statement : APPROVED FOR PUBLIC RELEASE