Accession Number : ADA254504


Title :   Mouse Model for Exoerythrocytic Stages of Plasmodium Falciparum Malaria Parasite,


Descriptive Note : Journal article


Corporate Author : WALTER REED ARMY INST OF RESEARCH WASHINGTON DC


Personal Author(s) : Sacci, Jr , John B ; Schriefer, Martin E ; Resau, James H ; Wirtz, Robert A ; Detolla, Jr, Louis J


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a254504.pdf


Report Date : May 1992


Pagination or Media Count : 7


Abstract : Research on the exoerythrocytic (EE) stages of human malaria parasites has been hindered because of the lack of an easily available suitable animal-mode. We report here an approach to produce mature EE-stage Plasmodium falciparum parasites by using severe combined immuno-deficient (scid) mice with transplanted human hepatocytes. Transplantation of human hepatocytes into scid mice (scid hu-hep), their subsequent intravenous infection with P. falciparum sporozoites, and the development of mature liver-stage macrozoites was achieved. Immunofluorescent staining of scid hu-hep kidney tissue sections demonstrated the presence of circumsporozoite protein (early during infection), merozoite surface antigen 1, and liver schizont antigen 1. The scid hu-hep model can serve as a source of human malaria liver-stage parasites, decreasing the need for nonhuman primates. Use of this model will facilitate characterization of EE- stage antigens and the assessment of stage-specific chemotherapeutic agents and candidate vaccines.


Descriptors :   *PARASITES , *MALARIA , *PLASMODIUM FALCIPARUM , MODELS , HUMANS , PROTEINS , REDUCTION , SURFACES , ANTIGENS , APPROACH , CHEMOTHERAPEUTIC AGENTS , PLASMODIUM , MEROZOITES , REPRODUCTION , KIDNEYS , TRANSPLANTATION , SPOROZOITES , AVAILABILITY , PLATES , COLORS , PRIMATES , MICE , LIVER


Subject Categories : Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE