Accession Number : ADA254496


Title :   Carboxylesterases of the Testes: Role In Activation of Toxicants.


Descriptive Note : Final rept. 1 Dec 88-31 May 90,


Corporate Author : AMERICAN COLL OF TOXICOLOGY BETHESDA MD


Personal Author(s) : Ventura, Alexandra


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a254496.pdf


Report Date : 31 May 1990


Pagination or Media Count : 5


Abstract : Organ specific distribution of carboxylesterases (Western blotting) was determined to be liver lung = testes = fat pancreas kidney. Carboxylesterase distribution among cell types of the testes was examined by in situ hybridization techniques. Results were inconclusive, as both the probe and the control hybridized to tissues macromolecules. More refinement of this techniques should provide better results. Other accomplishments include examination of the down-regulation of carboxylesterase levels by glucocorticoids. Apparently esterase levels are most dramatically down-regulated (approximately 6-fold) by dexamethasone phosphate (60 mg/kg x 5 days, i.p.) in the testes compared to the other tissues containing this enzyme.


Descriptors :   *LUNG , *MACROMOLECULES , *LIVER , *PANCREAS , *TESTES , CONTROL , PROBES , ACTIVATION , KIDNEYS , ESTERASES , CARBOXY LYASES , HYBRIDIZATION , PHOSPHATES , REGULATIONS , FATS , DISTRIBUTION , CELLS , ENZYMES


Subject Categories : Biology


Distribution Statement : APPROVED FOR PUBLIC RELEASE