Accession Number : ADA253974


Title :   Transfection of Murine and Human Hematopoietic Progenitors with Rearranged Immunoglobulin Genes,


Corporate Author : PROGRAM RESOURCES INC FREDERICK MD


Personal Author(s) : Kenney, James J


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a253974.pdf


Report Date : Jul 1992


Pagination or Media Count : 13


Abstract : There are three main objectives of the research conducted under the above contract. First to isolate hematopoietic stem cells in numbers adequate for transfection with rearranged immunoglobulin genes. Second, to develop techniques which will allow B cells expressing the transfected Ig-gene product to be activated by anti-idiotypic antibodies so that high levels of serum antibody are produced. Finally, to develop a human-mouse chimera that will allow us to transfect rearranged Ig-genes into human hematopoietic progenitors then grow and activate those cells in an animal model.


Descriptors :   *IMMUNOGLOBULINS , CHOLINES , HEMATOLOGY , GENES


Subject Categories : Biology


Distribution Statement : APPROVED FOR PUBLIC RELEASE