Accession Number : ADA239322


Title :   Salivary Platelet Activating Factor Levels in Periodontal Disease


Descriptive Note : Master's thesis,


Corporate Author : AIR FORCE INST OF TECH WRIGHT-PATTERSON AFB OH


Personal Author(s) : Garito, Martha L


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a239322.pdf


Report Date : May 1991


Pagination or Media Count : 89


Abstract : Platelet activating factor (PAF), a potent phospholipid inflammatory mediator is found in normal human saliva. This study sought to evaluate possible relationships between salivary PAF levels and periodontal disease. One ml of mixed saliva was collected from 69 untreated subjects presenting for evaluation at the UTHSC dental hygiene or periodontal clinic. After phospholipid extraction and fractionation by thin layer chromatography, salivary PAF activity was determined by platelet bioassay. PAF activity, estimated relative to that of authentic PAF (1-O-hexadecyl-2-acetyl-sn-glycero-3-phosphocholine (C16:O-AGEPC), was expressed in C16:O-AGEPC fmole equivalents/ml saliva; tracer amounts of H3- AGEPC included in all samples prior to initial extraction were used to calculate PAF recovery. Subjects were subdivided into 6 similarly sized groups according to disease severity (based on probing depths). The healthiest group, Gp 1, had or = 4 mm probing depths throughout, while the most severely affected group, Gp 6, averaged 4 mm probing depths in 50% of the sites. Correlation was found between the number of bleeding sites and the 6 groups. PAF levels generally increased from Gp 1 to Gp 6, with 1 levels (2,365 + or = 900 C16:O-AGEPC fmole equivalents/ml) being significantly lower than Gps 5 and 6 (10,489 + or = 1,775 and 10,251 + or = 3,075, respectively). The findings indicate that salivary PAF levels correlate with periodontal status and suggest that this phospholipid inflammatory mediator may play a role in the pathogenesis of periodontal disease.


Descriptors :   *ACTIVATION , SITES , DISEASES , SAMPLING , EXTRACTION , MIXING , OUTPATIENT CLINICS , POTENCY , DENTISTRY , BIOASSAY , HEMORRHAGE , BLOOD PLATELETS , SALIVA , PHOSPHOLIPIDS , THIN LAYER CHROMATOGRAPHY , ORAL HYGIENE , HUMANS


Subject Categories : Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE