Accession Number : ADA227442


Title :   Expression of the 'Bacillus anthracis' Protective Antigen Gene by Baculovirus and Vaccinia Virus Recombinants


Corporate Author : ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD


Personal Author(s) : Iacono-Connors, Lauren C ; Schmaljohn, Connie S ; Dalrymple, Joel M


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a227442.pdf


Report Date : Feb 1990


Pagination or Media Count : 7


Abstract : The gene encoding Bacillus anthracis protective antigen (PA) was modified by site-directed mutagenesis, subcloned into baculovirus and vaccinia virus plasmid transfer vectors (pAcYM1) and pSC-11, respectively), and inserted via homologous recombinations into baculovirus Autographa californica nuclear polyhedrosis virus or vaccinia virus (strains WR and Connaught). Expression of PA was detected in both systems by immunofluorescence assays with antisera from rabbits immunized with B. anthracis PA. Western blot (immunoblot) analysis showed that the expressed product of both systems was slightly larger (86 kilodaltons) than B. anthracis-produced PA (83.5 kilodaltons). Analysis of trypsin digests of virus-expressed and authentic PA suggested that the size difference was due to the presence of a signal sequence remaining with the virus-expressed protein.


Descriptors :   *ANTIBODIES , *IMMUNE SERUMS , VACCINIA VIRUS , RABBITS , SIGNALS , SEQUENCES


Subject Categories : Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE