Accession Number : ADA227358


Title :   Sulfur Mustard (SM) Lesions in Organ-Cultured Human Skin: Markers of Injury and Inflammatory Mediators


Descriptive Note : Final rept. 17 Feb 1987-16 Apr 1990


Corporate Author : JOHNS HOPKINS UNIV BALTIMORE MD SCHOOLOF HYGIENE AND PUBLIC HEALTH


Personal Author(s) : Dannenberg, Arthur M , Jr


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a227358.pdf


Report Date : 16 Apr 1990


Pagination or Media Count : 72


Abstract : The paranuclear vacuolization test for injury to human skin. Full thickness specimen of human skin (1.0 cm 2) were topically exposed to 10 yl of 0.03% to 1.0% SM and organ-cultured for 24 hr at 36 C. There was a straight-line dose-response relationship between the above concentrations of SM and the number of paranuclear vacuoles seen historically in the epidermis. Within the same SM dosage range, there was also a proportional decrease in 14 C-leucine incorporation by the explants. Early mediators of inflammation produced by SM in human skin. Culture fluids from SM-exposed and control full-thickness human skin explants contained similar amounts of angio-tensin-converting enzyme, tryspin- like and chymotryspin-like proteases, acid phosphatase, B-glucuronidase, B- galactosidase, lysozyme, deoxyribonuclease, ribonuclease, interleukin 1 and lactic dehydrogenase. However, the culture fluids from the SM-exposed explants contained increased amounts of histamine, plasminogen activator (PA), and usually, prostaglandin E2 (PGE2), compared to culture fluids from control explants. Keywords: Sulfur mustard, full-thickness human skin explants in organ culture; histology & histochemistry; Inflammatory medidators; Viability of human skin at 4 C.


Descriptors :   *MUSTARD AGENTS , *SULFUR , *SKIN(ANATOMY) , *LESIONS , ACTIVATION , HUMANS , DOSAGE , FLUIDS , WOUNDS AND INJURIES , MARKERS , RESPONSE(BIOLOGY) , INFLAMMATION , CULTURE , HISTOLOGY , EPIDERMIS , ORGANS(ANATOMY) , MURAMIDASE , ENZYME PRECURSORS , HISTAMINE , HISTOCHEMISTRY , ACID PHOSPHATASE , LACTIC DEHYDROGENASE , RIBONUCLEASE


Subject Categories : Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE