Accession Number : ADA223765


Title :   Flow Cytometric Analysis of Hepatocytes from Normal, PFDA, and PH/DEN/ PB-Treated Rats


Descriptive Note : Final rept.


Corporate Author : OHIO STATE UNIV RESEARCH FOUNDATION COLUMBUS


Personal Author(s) : Tarr, Melinda J ; Frazier, Donald E


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a223765.pdf


Report Date : 31 Dec 1989


Pagination or Media Count : 21


Abstract : The goal of this project was to provide preliminary data regarding the hepatotoxic and immunotoxic effects of perfluorodecanoic acid (PFDA). Flow cytometric evaluation of hepatocytes from PFDA-treated rats revealed an increase in size and granularity and a shift from tetraploidy towards diploidy, in rats treated with 50 mg/kg PFDA, as well as a suggestion of interference with lymphocyte blast transformation of splenocytes in these rats. These preliminary studies suggest that PFDA induces hepatocellular alterations which are detectable by flow cytometry, and induces both morphologic and possibly functional modulation of the immune system. Further studies are indicated to determine the significance of these findings. Keywords: Perfluorodecanoic ACID(PFDA); Hepatocarcinogenesis; Preneoplastic lesions; Flow cytometry; Immunotoxicity.


Descriptors :   *CELLS(BIOLOGY) , LIVER , MODULATION , LESIONS , LYMPHOCYTES , TRANSFORMATIONS , RATS , IMMUNITY


Subject Categories : Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE