Accession Number : ADA216819


Title :   Mechanisms of Cellular Membrane Effects of TCDD, Selected Perfluorinated Acids and Polyhalogenated Aromatic Hydrocarbons


Descriptive Note : Forecast rept.


Corporate Author : MICROBIOLOGICAL ASSOCIATES INC BETHESDA MD


Personal Author(s) : Rogers-Back, Andrea


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a216819.pdf


Report Date : 01 Feb 1985


Pagination or Media Count : 8


Abstract : The first six months of the contract have been devoted to examining the effects of perfluorinated acids and polyhalogenated aromatic hydrocarbons on the colony-forming ability of L5178Y cells. Two sub clones of this cell line have been utilized. One sub clone is designated L5178Y TK+/. The two cell lines differ markedly in their growth characteristics. L5178Y TK+/- cells tend to associate in clumps of cells and are grown in a shaker incubator to produce better cell suspensions. L5178Y TK+/+ cells grow as a single cell suspension without agitation. The toxic response of both cell lines after treatment with the perfluorinated acids (perfluoro-n-decanoic acid) was measured. In summary, the results for the perfluorinated acids with chain length of 9 or 10 indicate that there is some dissociation of colony growth from suspension growth. Medium type does not affect the toxicity. This would imply that these perfluorinated acids are producing toxicity through a membrane interaction. The dissociation appears to occur in the TK+/+ cells at concentrations approximately 20 higher than those previously reported. (aw)


Descriptors :   *ORGANIC ACIDS , *TOXICITY , *COLONIES(BIOLOGY) , *CLONES , *AROMATIC HYDROCARBONS , *HALOGENATED HYDROCARBONS , *CELLS(BIOLOGY) , FLUORINATION , CONCENTRATION(CHEMISTRY) , DISSOCIATION , CELL DIVISION , RESPONSE , POLYMERS , DECANES , MEMBRANES(BIOLOGY) , INTERACTIONS


Subject Categories : Toxicology


Distribution Statement : APPROVED FOR PUBLIC RELEASE