Accession Number : ADA213649


Title :   Primary Events in Olfactory Receptiom


Descriptive Note : Final rept. 1 Aug 1986-31 Jul 1989


Corporate Author : DUKE UNIV MEDICAL CENTER DURHAM NC DEPT OF NEUROBIOLOGY


Personal Author(s) : Anholt, Robert R


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a213649.pdf


Report Date : 10 Oct 1989


Pagination or Media Count : 10


Abstract : The following major discoveries are reported on herein: 1 We have established procedures for the dissociation and reassembly of ciliary membranes from olfactory epithelium under conditions that allow survival of the olfactory adenylate cyclase. These studies represent a first step toward the molecular dissection and functional reconstitution of the chemosensory membrane. 2 We have discovered that the olfactory adenylate cyclase is regulated by calcium/ calmodulin and characterized the effects of calcium/calmodulin on modulation of this enzyme. 3 We have characterized proteins that are substrates for cyclic AMP-dependent protein kinase in ciliary and axonal membranes of olfactory receptor cells. In addition, we have identified a protein of which the phosphorylation proceeds independent of cyclic nucleotides and is inhibited by low concentrations of calcium. 4 We have established a library of monoclonal antibodies against olfactory cilia and identified several epitopes that are unique to chemosensory cilia and absent from olfactory nerve membranes, respiratory cilia and membranes from a host of other tissues. Keywords: Olfaction, Smell, Cyclic AMP, Signal transduction, Monoclonal antibodies.


Descriptors :   *OLFACTORY NERVE , *SMELL , *NERVE TRANSMISSION , *SENSE ORGANS , *ADENYL CYCLASE , PROTEINS , SUBSTRATES , SURVIVAL(GENERAL) , SIGNALS , LOW LEVEL , CONCENTRATION(COMPOSITION) , RESPIRATORY SYSTEM , TRANSDUCERS , MONOCLONAL ANTIBODIES , MODULATION , DISSOCIATION , LIBRARIES , CYCLIC COMPOUNDS , ADENOSINE PHOSPHATES , NUCLEOTIDES , PHOSPHORYLATION , NERVE FIBERS , CILIA , DISSECTION , ENZYMES , CELLS , MOLECULES , MEMBRANES(BIOLOGY) , CALCIUM , EPITHELIUM


Subject Categories : Genetic Engineering and Molecular Biology
      Biology


Distribution Statement : APPROVED FOR PUBLIC RELEASE