Accession Number : ADA213230


Title :   Altered Signal Transduction in Renal Cell Injury Following Hemorrhagic Shock or Anoxia


Descriptive Note : Annual rept. Jul 1988-Jul 1989


Corporate Author : MARYLAND UNIV BALTIMORE DEPT OF PATHOLOGY


Personal Author(s) : Trump, Benjamin F ; Berezesky, Irene K ; Smith, Mary W ; Anthony, Ronald


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a213230.pdf


Report Date : 01 Jul 1989


Pagination or Media Count : 166


Abstract : Our objective in this project is to delineate the relationship between transmembrane signalling; ion deregulation, e.g., Ca2+i, H+i, and Mg2+i; cell injury; inflammation; and repair. Our laboratory has been engaged in this type of work for some years and have recently reviewed the subject (Trump et al., 1984; Trump and Berezesky, 1985). The achievement of this objective will have important implications for the prevention, diagnosis, and treatment of shock, trauma, related cell injury, and cell death. This project is aimed at the delineation of the relationship between ion deregulation e.g., Ca2+i, H+i, Na+i, K+i, and Mg2+i, cell injury, signal transduction, altered DNA synthesis, and gene expression as they relate to cell death, regeneration, inflammation, and repair in the rat and human proximal tubule epithelium (PTE). Techniques utilized include electron microscopy; phase and Nomarski light microscopy; digital imaging fluorescence microscopy (DIFM) and video microscopy, image analysis, and photon counting; fluorescent probes, e.g., BCECF, rhodamine 123, and propidium iodide; assays of the PI pathway including IP3 and DAG; assays of protein kinase C; and measurements of mRNA modulation of c-fos,. c-myc, and c-jun. Keywords: Ion deregulation; Calcium; PH; Cell injury; Signal transduction; DNA synthesis; Gene expression; Proximal tubule epithelium; Anoxia; Hemorrhagic shock; Ischemia. (kt)


Descriptors :   *ANOXIA , *ISCHEMIA , *HEMORRHAGIC SHOCK , *NERVE TRANSMISSION , FLUORESCENCE , RATS , CELLS , COUNTING METHODS , LIGHT , DEOXYRIBONUCLEIC ACIDS , MICROSCOPY , ELECTRON MICROSCOPY , REPAIR , SIGNALS , SHOCK , IMAGES , TUBULAR STRUCTURES , PHOTONS , GENES , TRANSDUCERS , WOUNDS AND INJURIES , DEATH , VIDEO SIGNALS , CELLS(BIOLOGY) , INFLAMMATION , BIOSYNTHESIS , TRAUMA , EPITHELIUM , PROBES , CALCIUM , IMAGE PROCESSING , DIGITAL SYSTEMS


Subject Categories : Biochemistry
      Genetic Engineering and Molecular Biology


Distribution Statement : APPROVED FOR PUBLIC RELEASE