Accession Number : ADA211335


Title :   Oxidative Damage in the Guinea Pig Hippocampal Slice


Corporate Author : ARMED FORCES RADIOBIOLOGY RESEARCH INST BETHESDA MD


Personal Author(s) : Pellmar, Terry C ; Neel, Kathryn L


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a211335.pdf


Report Date : Jan 1989


Pagination or Media Count : 7


Abstract : Free radicals and active oxygen compounds are implicated in brain ischemia and head trauma. Previous studies have shown that free radical, generated by radiation and through the Fenton reaction, produced both synaptic and postsynaptic damage in the hippocampal brain slice. To evaluate the contribution of oxidation to the observed damage, the actions of the oxidants, chloramine-T and N-chlorosuccinimide (NCS), were studied on electrophysiological responses in the hippocampal slice isolated from the brains of guinea pigs. Electrical stimulation of afferents to neurons of the CA1 region of hippocampus evoked a population postsynaptic potential (population PSP) in the dendritic layer and a population spike in the cell body layer. These studies suggest that oxidation reactions can account for the synaptic component of the damage produced by free radicals but can not account for the postsynaptic effects. Keywords: Chloramine-T, N-chlorosuccinimide, Oxidation; Free radical; Hippocampus. Reprints.


Descriptors :   *BRAIN , *ISCHEMIA , *OXYGEN COMPOUNDS , *HIPPOCAMPUS , *SYNAPSE , *FREE RADICALS , OXIDATION , OXIDIZERS , NERVE CELLS , ELECTRIC CURRENT , RESPONSE(BIOLOGY) , HEAD(ANATOMY) , DENDRITIC STRUCTURE , TRAUMA , OXIDATION REDUCTION REACTIONS , ELECTROPHYSIOLOGY , GUINEA PIGS , POPULATION , CELLS , LAYERS , DAMAGE , STIMULATION(GENERAL) , REPRINTS , RADIATION


Subject Categories : Biochemistry
      Anatomy and Physiology


Distribution Statement : APPROVED FOR PUBLIC RELEASE