Accession Number : ADA210399


Title :   Molecular Biology and Physiology of Methanogenic Archaebacteria


Descriptive Note : Annual rept. Jul 1988-Jun 1989


Corporate Author : OKLAHOMA UNIV NORMAN DEPT OF BOTANY AND MICROBIOLOGY


Personal Author(s) : Nagle, Jr , D P ; McCarthy, D ; Tanner, R S


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a210399.pdf


Report Date : 27 Jun 1989


Pagination or Media Count : 17


Abstract : Methane-producing archaebacteria are worthy of their novel biology and potential in anaerobic bioprocessing. This work continues to study the biochemistry, genetics, and molecular biology of the thermophilic autotroph Methanobacterium thermoautotrophicum. DNA from antimetabolite-resistant mutant strains was used to transform sensitive recipient cells to resistance, and DNA was cloned into Escherichia coli plasmids. This DNA will be mutated with transposons in the E. coli host, then isolated and used to transform methanogen cells to selectable mutant phenotypes. Mutant strains resistant to purine analogs were used to determine that wild type cells of M. thermoautotrophicum possess an almost complete set of enzymes for uptake, activation, and interconversion of purine bases and nucleosides. These mutants and the information about the pathways will be the basis for generating a genetic map. Metabolic studies of a unique formate auxotroph revealed a new role for this one carbon compound in the anabolic metabolism of this methanogen.


Descriptors :   *BACTERIA , *BIOCHEMISTRY , *MOLECULAR BIOLOGY , *GENETICS , *PHYSIOLOGY , ACTIVATION , ENZYMES , METABOLISM , MUTATIONS , ESCHERICHIA COLI , GENES , NUCLEOSIDES , CHEMICAL ANALOGS , PURINES , FORMATES , CELLS(BIOLOGY) , CLONES , MAPS , STRAINS(BIOLOGY) , CHROMOSOMES , DEOXYRIBONUCLEIC ACIDS , CARBON , RESISTANCE , BIOLOGY


Subject Categories : Biochemistry
      Genetic Engineering and Molecular Biology
      Microbiology


Distribution Statement : APPROVED FOR PUBLIC RELEASE