Accession Number : ADA202780


Title :   Effects of Simultaneous Radiofrequency Radiation and Chemical Exposure of Mammalian Cells. Volume 2


Descriptive Note : Final rept. 2 Jan 1984-31 Dec 1986


Corporate Author : TEXAS UNIV HEALTH SCIENCE CENTER AT SANANTONIO DEPT OF RADIOLOGY


Personal Author(s) : Meltz, Martin L ; Ciaravino, Victor ; Kerbacher, James J ; Eagan, Phyllis


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a202780.pdf


Report Date : Jul 1988


Pagination or Media Count : 84


Abstract : A circulating water-bath exposure system has been designed for in vitro radiofrequency radiation (RFR) exposure studies in the 915 to 2450 MHz range. A continuously rotating Styrofoam float, holding ten T-25 tissue culture flasks, averages out field heterogeneity and allows mixing in the medium in the flasks. The presence of cells in the exposure flask (as attached monolayer or cell suspension) did not result in an SAR different from that measured in the medium without cells present. We have posed two hypotheses relative to the mutagenic activity of RFR: (a) that RFR by itself is genotoxic to mammalian cells in vitro; and (b) that a simultaneous exposure of mammalian cells to RFR during treatment with a genotoxic chemical will result in an alteration of the genotoxic activity of the chemical alone. RFR exposure alone (at moderate power levels which resulted in a temperature increase in the cell culture medium of no more than 3 C) is not mutagenic. during simultaneous treatment, the RFR does not affect either the inhibition of cell growth or the extent of mutagenesis resulting from the chemical treatment alone. The same two hypotheses were explored for induction of sister chromatid exchanges (SCEs) and chromosome aberrations in Chinese hamster ovary cells which were similarly exposed to RFR and chemicals. The chemicals studied for SCE induction were mitomycin C (MMC) and Adriamycin. Keywords: Microwaves.


Descriptors :   *RADIATION EFFECTS , POWER LEVELS , RADIATION , EXPOSURE(GENERAL) , MAMMALS , CHEMICALS , LAYERS , CELLS , MICROWAVES , MUTATIONS , CHROMOSOMES , IN VITRO ANALYSIS , CHEMICAL REACTIONS , HETEROGENEITY , SYNCHRONISM , HYPOTHESES , EXPOSURE(PHYSIOLOGY) , MUTAGENS , OVARIES , FLASKS , CHROMATIN , CHEMOTHERAPEUTIC AGENTS , CULTURE MEDIA , INHIBITION , CELLS(BIOLOGY) , RADIOFREQUENCY , CULTURES(BIOLOGY) , HAMSTERS , TOXINS AND ANTITOXINS , CHINA


Subject Categories : Radiobiology
      Toxicology


Distribution Statement : APPROVED FOR PUBLIC RELEASE