Accession Number : ADA195969


Title :   Lipopolysaccharide Antigens of Pseudomonas aeruginosa and Design of Novel Vaccines.


Descriptive Note : Final rept. (Annual) 1 Sep 84-30 Apr 86,


Corporate Author : OHIO STATE UNIV RESEARCH FOUNDATION COLUMBUS


Personal Author(s) : Horton, Derek


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a195969.pdf


Report Date : Sep 1987


Pagination or Media Count : 28


Abstract : The antigenic lipopolysaccharides (LPS) of the cell coats of five of the seven Fisher immunotype strains of the Gram-negative pathogen Pseudomonas aeruginosa have been characterized structurally by chemical methods involving degradation techniques, and by physical methods, notably high-field NMR spectroscopy. Of the seven Fisher types, the O-Chain structures of five of the types have been characterized in our laboratories. Partial structures for the remaining two types have been elucidated. The Gram-negative bacterium Pseudomonas aeruginosa is ubiquitous in its occurrence and is characterized by a very wide variety of strains as determined by conventional serotyping methods. Although healthy individuals normally have a high innate resistance to infection by Pseudmonas aeruginosa as a result of effective combat by matured antibodies, the organism is an opportunistic pathogen; virulent strains lead to dangerous, often fatal, infections in human subjects whose natural resistance has been lowered. Conventional treatment with antibiotics effective against Gram-positive organisms is of little value, as such therapeutic agents are unable to penetrate the lipopolysaccharide layer that forms a thick, hydrophobic outer coating of the cells in Gram-negative organisms. In view of the limited effectiveness of conventional antibiotic agents for combat of Pseudomonas infections, immunotherapeutic methods have been explored as an alternative method. Keywords: Burns; cystic fibrosis. (kt)


Descriptors :   ANTIBIOTICS , ANTIBODIES , ANTIGENS , BACTERIA , CHEMICALS , CHEMOTHERAPEUTIC AGENTS , COATINGS , CYSTS , DEGRADATION , DISEASES , EXTERNAL , FIBROSIS , GRAM NEGATIVE BACTERIA , HYDROPHOBIC PROPERTIES , INFECTIOUS DISEASES , LAYERS , LIPOPOLYSACCHARIDES , PHYSICAL PROPERTIES , PSEUDOMONAS , PSEUDOMONAS AERUGINOSA , RESISTANCE , SPECTROSCOPY , STRAINS(BIOLOGY) , STRUCTURES , THICKNESS , VACCINES , VIRULENCE , WARFARE


Subject Categories : Microbiology


Distribution Statement : APPROVED FOR PUBLIC RELEASE