Accession Number : ADA193265


Title :   Liquid Storage at 4 deg C of Previously Frozen Red Cells


Descriptive Note : Journal article


Corporate Author : LETTERMAN ARMY INST OF RESEARCH PRESIDIO OF SAN FRANCISCO CA


Personal Author(s) : Moore, G L ; Ledford, M E ; Mathewson, P J ; Hankins, D J


Report Date : Dec 1987


Pagination or Media Count : 3


Abstract : Fresh human blood was collected in citrate-phosphate-dextrose, frozen by a high-glycerol technique, and stored at -80 deg C. The red cells ere thawed, deglycerolized, and resuspended in a final was solution, ADSOL (Fenwal Laboratories), or an additive solution (AS) containing glucose, adenine, mannitol, and phosphate. The cells were then stored at 4 to 6 deg C for 21 days and assayed weekly for adenosine triphosphate and 2,3 diphosphoglycerate, pH, glucose use, and lysis. AS and, to a lesser extent, ADSOL produced metabolic profiles similar to or better than profiles of cells not frozen and stored in commercially available additive solutions. AS offers a potential post-thaw preservative solution for red cells that would greatly increase the flexibility and reduce the expense of using frozen blood. A sterile post-thaw storage capability will make the stockpiling of frozen red cells a practical concept for both military and civilian blood banks.


Descriptors :   *BLOOD STORAGE , ACID CITRATE DEXTROSE , ADDITIVES , ADENINE , ADENOSINE PHOSPHATES , BLOOD , BLOOD BANKS , BLOOD CHEMISTRY , CIVILIAN PERSONNEL , COSTS , ERYTHROCYTES , FREEZING , GLUCOSE , HUMANS , LYSIS , MANNITOL , METABOLISM , PHOSPHATES , REPRINTS , SOLUTIONS(MIXTURES) , STOCKPILES


Subject Categories : Medical Facilities, Equipment and Supplies


Distribution Statement : APPROVED FOR PUBLIC RELEASE