Accession Number : ADA192855


Title :   Production and Characterization of Monoclonal Antibodies Against the Protective Antigen Component of Bacillus anthracis Toxin


Corporate Author : ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD


Personal Author(s) : Little, Stephen F ; Leppla, Stephen H ; Cora, Elsa


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a192855.pdf


Report Date : 21 Jan 1988


Pagination or Media Count : 34


Abstract : Thirty-six monoclonal antibodies to the protective antigen protein of Bacillus anthracis exotoxin have been characterized for affinity, antibody sub- type, competitive binding to antigenic regions, and ability to neutralize the lethal and edema toxin activities. At least 23 antigenic regions were detected on protective antigen by a blocking, enzyme-linked immunosorbent assay. Two clones, 3B6 and 14B7, competed for a single antigenic region and neutralized the activity of both the lethal toxin in vivo (Fisher 344 rat) and the edema toxin in vitro (CHO cells). These two antibodies blocked the binding of iodine 125-PA to FRL-103 cells. Our results support the proposal that binding of protective antigen to cell receptors is required for expression of toxicity.


Descriptors :   *ANTIBODIES , *CLONES , *VACCINES , *EXOTOXINS , *BACILLUS ANTHRACIS , PRODUCTION , TOXICITY , ENZYMES , PROTEINS , IN VITRO ANALYSIS , REGIONS , LETHALITY , MICE , IN VIVO ANALYSIS , IMMUNOASSAY , ANTIGENS , IMMUNIZATION , BACTERIAL TOXINS , ANTIGEN ANTIBODY REACTIONS , EDEMA


Subject Categories : Medicine and Medical Research
      Pharmacology


Distribution Statement : APPROVED FOR PUBLIC RELEASE