Accession Number : ADA191862


Title :   Determination of the In Vitro and In Vivo Activity of Compounds Tested Against Punta Toro Virus.


Descriptive Note : Annual rept. 1 Dec 86-30 Nov 87,


Corporate Author : UTAH STATE UNIV LOGAN


Personal Author(s) : Sidwell, Robert W


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a191862.pdf


Report Date : 29 Dec 1987


Pagination or Media Count : 169


Abstract : This report describes the second year's studies using Punta Toro virus (PTV) infections in vitro and in vivo as test systems for evaluating anti-PTV compounds. A large plaque-purified pool of Adames strain of PTV was studied to attempt to increase the lethal infectivity of the virus for C57BL/6 mice by passing the virus through mice, using pooled serum taken 2 days after infection to prepare a new PTV pool. Studies were run to confirm the acceptability of the mice which are used for our anti-PTV experiments. Preliminary assessments of toxicity of AVs compounds in 3-4 week-old mice were run on 30 compounds, using death and host weight change as parameters. In vitro anti-PTV evaluations were performed on 295 AVS compounds in LLC-MK2 cells with inhibition of viral cytopathic effect (CPE) as initial endpoint and reduction of virus titer (VTR) at maximum tolerated doses of initially active test compounds used as a confirmatory antiviral endpoint. Twenty-three AVS compounds inhibitory to Adames PTV in vitro were also evaluated against the Balliet strain of PTV. Death and mean survival time were used as initial evaluation parameters; reduction in liver icterus, serum glutamic oxalic acid transaminase, serum glutamic pyruvic acid transaminase, serum virus and liver virus were also used in confirming in vivo anti-PTV experiments.


Descriptors :   *VIRUSES , *ANTIVIRAL AGENTS , ACCEPTABILITY , BLOOD SERUM , DEATH , DOSAGE , IN VITRO ANALYSIS , IN VIVO ANALYSIS , INFECTIOUS DISEASES , INHIBITION , LIVER , MEAN , MICE , PATHOLOGY , SURVIVAL(GENERAL) , TEST AND EVALUATION , TIME , TOXICITY , LETHALITY , BODY WEIGHT


Subject Categories : Pharmacology
      Microbiology


Distribution Statement : APPROVED FOR PUBLIC RELEASE