Accession Number : ADA179862


Title :   Development of a DNA-Based Method for Distinguishing the Malaria Vectors, Anopheles gambiae From Anopheles arabiensis.


Descriptive Note : Annual rept. 15 Jun 85-14 Jun 86,


Corporate Author : EMORY UNIV ATLANTA GA DEPT OF BIOLOGY


Personal Author(s) : Finnerty,Victoria


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a179862.pdf


Report Date : Jun 1986


Pagination or Media Count : 31


Abstract : The Anopheles gambiae complex includes six morphologically identical species, two of which (A. gambiae and A. arabiensis) are the primary African malaria vectors today. Since two or more of the species are commonly sympatric, epidemiological studies to determine the involvement of each in malaria transmission have been difficult. This report describes our efforts to identify a DNA segment from A. gambiae which when used as a probe on Southern blots containing both A. gambiae and A. arabiensis DNA will reveal a restriction fragment length polymorphism. These diagnostic bands are consistently seen on blots of whole genomic DNA. The method can also be used on dried single mosquitoes or mosquito abdomens although the DNA extraction method currently used does not always yield sufficient DNA for the test. Keywords: Clones; Assaying; Culicidae; Autoradiography; Hybridization.


Descriptors :   *DEOXYRIBONUCLEIC ACIDS , *ANOPHELES , BANDS(STRIPS) , TRANSMITTANCE , DIAGNOSIS(MEDICINE) , CLONES , MALARIA , AFRICA , ASSAYING , EPIDEMIOLOGY , DRYING , DISEASE VECTORS , AUTORADIOGRAPHY , CULICIDAE , HYBRIDIZATION


Subject Categories : Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE