Accession Number : ADA173061
Title : Cellular Effects of Perfluorinated Fatty Acids.
Descriptive Note : Final rept. 1 Jan 83-31 Dec 84,
Corporate Author : CALIFORNIA UNIV IRVINE
Personal Author(s) : Berns,Michael W
Report Date : Jan 1985
Pagination or Media Count : 15
Abstract : The compound perfluoro-n-decanoic acid (PFDA) was exposed to three tissue culture cell lines, PTK2 (kidney), BRL (buffalo rat liver) and K9 (clone 9, rat liver) in concentrations up to 200 micrograms/ml for 24 hr. time periods. The technique of fluorescence recovery after photobleaching (FRAP) was used ot examine cell membrane fluidity, specifically with respect to the membrane lipids. Results demonstrated that PFDA affects the fluidity of both liver lines but not the kidney line. Furthermore, differential sensitivity was observed between the responsiveness of the two liver lines. (BRL was more sensitive than K9.) The increased PFDA-induced membrane fluidity was transient: recovery occured by 48 hr following removal of the PFDA. The FRAP technique appears useful in screening agents for membrane effects as well as elucidating mechanisms of agent action.
Descriptors : *LIVER , *CELLS(BIOLOGY) , *KIDNEYS , *FATTY ACIDS , TIME INTERVALS , MEMBRANES(BIOLOGY) , RECOVERY , TISSUE CULTURE CELLS , FLUORESCENCE , RATS , LIPIDS , SENSITIVITY , MEMBRANES , FLUIDS , VISCOSITY , FLUORINATION , DIFFERENTIAL CROSS SECTIONS
Subject Categories : Biochemistry
Anatomy and Physiology
Distribution Statement : APPROVED FOR PUBLIC RELEASE