Accession Number : ADA172671


Title :   Radioprotective Properties of Detoxified Lipid A from Salmonella minnesota R595


Corporate Author : ARMED FORCES RADIOBIOLOGY RESEARCH INST BETHESDA MD


Personal Author(s) : Snyder, Stephen L ; Walden, Thomas L ; Patchen, Myra L ; MacVittie, Thomas J ; Fuchs, Pinhas


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a172671.pdf


Report Date : Jan 1986


Pagination or Media Count : 8


Abstract : In the past, the toxicity of bacterial lipopolysaccharide (LPS) or its principal bioactive component, lipid A, has detracted from their potential use as radioprotectants. Recently, a relatively nontoxic monophosphoryl Lipid A (LAM) that retains many of the immunobiologic properties of LPS has been isolated from a polysaccharide deficient Re mutant strain of Salmonella minnesota (R595). The ability of the native endotoxic goycolipid (GL) from S. minnesota (R595) as well as diphosphoryl lipid A (LAD) and nontoxic monophosphoryl lipid A (LAM) derived from GL to protect LPS responsive (CD2F1 or C3H/HeN) and nonresponsive (C3H/HeJ) mice from cobalt 60 gamma irradiation has been studied. Administration of GL, LAD, or LAM to CD2F1 or C3H/HeN mice (400 micrograms/kg) 24 h prior to exposure provided significant radioprotection. No protection was afforded to C3H/HeJ mice. Experiments were also conducted to determine the relative abilities of GL, LAD, and LAM to stimulate hematopoiesis as reflected by the endogenous spleen colony (E-CFU) assay. Protection was not correlated with the ability of these substances to increase E-CFUs or to induce colony-stimulating activity (CSA).


Descriptors :   *LIPIDS , *RADIOPROTECTIVE AGENTS , *SALMONELLA , MICE , IMMUNOLOGY , BACTERIAL TOXINS , ENDOTOXINS , SPLEEN , COBALT , BIOASSAY , LIPOPOLYSACCHARIDES , HEMATOPOIESIS , IRRADIATION , GAMMA RAYS , REPRINTS , RADIOACTIVE ISOTOPES


Subject Categories : Pharmacology


Distribution Statement : APPROVED FOR PUBLIC RELEASE