Accession Number : ADA166957


Title :   Collaborative Study of Daphnia magna Static Renewal Assays.


Descriptive Note : Final rept. Dec 82-Dec 85,


Corporate Author : SPRINGBORN BIONOMICS INC WAREHAM MA*


Personal Author(s) : Bentley,R E ; Surprenant,D C ; Petrocilli,S R


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a166957.pdf


Report Date : Jan 1986


Pagination or Media Count : 311


Abstract : A Daphnia magna chronic test was evaluated as to its suitability as a hazardous waste testing and assessment procedure. The purpose of this project was to develop a practical and technically valid Daphnia magna chronic test protocol and then to verify the accuracy and precision of this protocol in collaborative studies conducted jointly in governmental, academic, industrial and contract testing laboratories. Based on the results of this collaborative testing, the verified test protocol would serve as a new standard test method. Results (both intra- and interlaboratory testing) indicated a high degree of accuracy and precision for routine tests with daphnids as the test organism. Results illustrated those effect criterion which were sensitive and reproducible measures of toxic effects and those which were not. It was also determined that environmental testing laboratories differed substantially in their individual conformance to EPA Good Laboratory Practice requirements. In summary, this study resulted in the development and validation of a technically credible protocol which when employed by competent laboratory personnel, produces reliable and useful data for evaluating the potential environmental hazard of solid waste leachates or other toxic mixtures. Keywords: Copper, Sodium pentachlorophenate, Toxicity.


Descriptors :   *TEST METHODS , *BIOASSAY , *DAPHNIA , REQUIREMENTS , LABORATORY TESTS , ENVIRONMENTS , HIGH RATE , HAZARDS , VALIDATION , LIQUIDS , TOXICITY , ACCURACY , SENSITIVITY , MIXTURES , COPPER , STANDARDIZATION , ENVIRONMENTAL TESTS , WASTES , SOLID WASTES , REPRODUCIBILITY , LEACHING , HAZARDOUS MATERIALS


Subject Categories : Civil Engineering


Distribution Statement : APPROVED FOR PUBLIC RELEASE