Accession Number : ADA157237


Title :   Spectral 'Fingerprinting' of Phytoplankton Populations by Two-Dimensional Fluorescence and Fourier-Transform-Based Pattern Recognition.


Descriptive Note : Interim technical rept.,


Corporate Author : EMORY UNIV ATLANTA GA DEPT OF CHEMISTRY


Personal Author(s) : Oldham,P B ; Zillioux,E J ; Warner,I M


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a157237.pdf


Report Date : 08 Jul 1985


Pagination or Media Count : 34


Abstract : The selectivity of fluorescence spectroscopy is exploited for the characterization of marine algae. Two-dimensional, digital images of in vivo fluorescence intensity versus excitation and emission wavelengths, called excitation-emission matrices (EEMs), are used as spectral fingerprints for marine phytoplankton populations. Fourier-transform-based pattern recognition is described along with its inherent strengths and weaknesses for the analysis of natural populations. The EEMs of unknown algae are compared to a library of standard EEMs representing 23 algal species and 6 classes with better than 80% accuracy. The EEMs acquired under different physiological conditions are used in determining pattern recognition reliability. The potential for fingerprinting mixed populations and oceanographic regions is also discussed. Originator supplied keywords include: Fluorescence analysis; Marine analysis; Fourier data analysis; Pattern recognition; Chlorophyll fluorescence; Spectral matching.


Descriptors :   *FLUORESCENCE , *PATTERN RECOGNITION , *ALGAE , *CHLOROPHYLLS , FOURIER TRANSFORMATION , FREQUENCY , DATA PROCESSING , DIGITAL SYSTEMS , EMISSION , SPECTROSCOPY , TWO DIMENSIONAL , EXCITATION , INTENSITY , POPULATION , IDENTIFICATION , RELIABILITY , SPECTRA , IMAGES , PHYSIOLOGICAL EFFECTS , MARINE BIOLOGY , MIXING , FLUOROMETERS , IN VIVO ANALYSIS , MATCHING , LIBRARIES , FOURIER ANALYSIS , PHYTOPLANKTON , MULTICHANNEL , OCEANOGRAPHY , SPECTRUM SIGNATURES , FINGERPRINTS


Subject Categories : Biological Oceanography
      Test Facilities, Equipment and Methods


Distribution Statement : APPROVED FOR PUBLIC RELEASE