Accession Number : ADA147175


Title :   Cellular Biochemistry and Cytogenetics in a Rat Lung Tumor Model


Descriptive Note : Annual rept. 1 Jul 1983-30 Jun 1984


Corporate Author : CALIFORNIA UNIV IRVINE DEPT OF COMMUNITY AND ENVIRONMENTAL MEDICINE


Personal Author(s) : Rasmussen, R E ; Fong, A T


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a147175.pdf


Report Date : Oct 1984


Pagination or Media Count : 23


Abstract : Two projects are underway in parallel in this subprogram. The first uses the rat lung tumor system in which squamous cell carcinomas can be induces by polycyclic aromatic hydrocarbon (PAH) such as 3-methylcholanthrene or benzo(a)pyrene (BaP). The second project employs a respiratory carcinogen model in which tumors can be induced in the Syrian hamster respiratory tract by administration of diethylnitrosamine (DEN). For the rat lung tumor system the specific aims are: (1) to conduct studies of the the effect of 3- methylcholanthrene (MCA) on DNA synthesis and cell proliferation in the rat lung; (2) to treat rats intratracheally with MCA and asbestos and measure effects on cell proliferation as this may relate to tumorigenesis; and (3) to separate lung cells from rats and measure their metabolism of 3H-BaP in vitro. For the hamster DEN lung tumor system the specific aims are: (1) in the hamster model system, to measure formation of 7-ethylguanine (7-EtG) and O6-ethylguanine (6-EtG) during acute and chronic treatment with DEN; and (2) to study cell proliferation in the hamster lung and liver after acute or chronic exposure to DEN.


Descriptors :   *NEOPLASMS , RATS , METABOLISM , CELLS(BIOLOGY) , HAMSTERS , ANATOMICAL MODELS , CYTOLOGY , METABOLITES , GENETICS , LUNG , BIOCHEMISTRY


Subject Categories : Biochemistry
      Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE